Research Article
Abstract
This
study investigated the impact of ethanol extract (EEIG) and ethyl acetate
fraction (EAF) of Irvingia gabonensis seeds on reproductive hormones,
oxidative, inflammatory and apoptotic markers in Wistar rats subjected to lead
acetate-induced reproductive damage. The research sought to elucidate the
methods by which I. gabonensis mitigates male reproductive toxicity. Forty-five
male Wistar rats, aged 8 to 10 weeks, were categorised into nine groups as
follows: Group I was administered 1 ml/kg of distilled water (control) for 56
days; Group II was administered 60 mg/kg of lead acetate for 28 days. Groups
III, IV, and V were administered 60 mg/kg of lead acetate during the initial 28
days, succeeded by 125 mg/kg, 250 mg/kg, and 500 mg/kg of EEIG, respectively,
for an additional 28 days. Groups VI, VII, and VIII were administered 60 mg/kg
of lead acetate for 28 days, thereafter receiving 50 mg/kg, 100 mg/kg, and 200
mg/kg of EAF, respectively, for an additional 28 days. Group IX was
administered 60 mg/kg of lead acetate for a duration of 28 days, thereafter
receiving 1 ml/kg of distilled water. All therapies were delivered orally.
Measured parameters encompassed FSH, LH, testosterone, SOD, MDA, 8OHdG, TNF-α,
Caspase-3 and Bcl-2, utilizing established methodologies. The results indicated
substantial decreases in FSH, LH, testosterone, SOD, Bcl-2 levels in the lead
acetate group, accompanied by elevated levels of MDA, 8OHdG, TNF-α, and Caspase
3. The administration of EEIG and EAF counteracted these alterations, with
increased dosages demonstrating enhanced restoration of hormone levels and
reduced oxidative and inflammatory indicators. This study demonstrates that Irvingia
gabonensis possesses antioxidative, anti-inflammatory, and anti-apoptotic
properties, effectively mitigating lead acetate-induced reproductive toxicity
in male Wistar rats. Both ethanol extract and ethyl acetate fraction showed
dose-dependent efficacy in restoring reproductive hormones, reducing oxidative
stress, and modulating inflammatory and apoptotic markers. These findings
suggest I. gabonensis as a potential natural therapy for male
reproductive damage caused by environmental toxins, warranting further
exploration of its clinical relevance and mechanisms.
Abstract Keywords
Irvingia gabonensis, lead toxicity,
oxidative stress, antioxidant, hormonal balance, male infertility, DNA fragmentation.
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This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
This
study investigated the impact of ethanol extract (EEIG) and ethyl acetate
fraction (EAF) of Irvingia gabonensis seeds on reproductive hormones,
oxidative, inflammatory and apoptotic markers in Wistar rats subjected to lead
acetate-induced reproductive damage. The research sought to elucidate the
methods by which I. gabonensis mitigates male reproductive toxicity. Forty-five
male Wistar rats, aged 8 to 10 weeks, were categorised into nine groups as
follows: Group I was administered 1 ml/kg of distilled water (control) for 56
days; Group II was administered 60 mg/kg of lead acetate for 28 days. Groups
III, IV, and V were administered 60 mg/kg of lead acetate during the initial 28
days, succeeded by 125 mg/kg, 250 mg/kg, and 500 mg/kg of EEIG, respectively,
for an additional 28 days. Groups VI, VII, and VIII were administered 60 mg/kg
of lead acetate for 28 days, thereafter receiving 50 mg/kg, 100 mg/kg, and 200
mg/kg of EAF, respectively, for an additional 28 days. Group IX was
administered 60 mg/kg of lead acetate for a duration of 28 days, thereafter
receiving 1 ml/kg of distilled water. All therapies were delivered orally.
Measured parameters encompassed FSH, LH, testosterone, SOD, MDA, 8OHdG, TNF-α,
Caspase-3 and Bcl-2, utilizing established methodologies. The results indicated
substantial decreases in FSH, LH, testosterone, SOD, Bcl-2 levels in the lead
acetate group, accompanied by elevated levels of MDA, 8OHdG, TNF-α, and Caspase
3. The administration of EEIG and EAF counteracted these alterations, with
increased dosages demonstrating enhanced restoration of hormone levels and
reduced oxidative and inflammatory indicators. This study demonstrates that Irvingia
gabonensis possesses antioxidative, anti-inflammatory, and anti-apoptotic
properties, effectively mitigating lead acetate-induced reproductive toxicity
in male Wistar rats. Both ethanol extract and ethyl acetate fraction showed
dose-dependent efficacy in restoring reproductive hormones, reducing oxidative
stress, and modulating inflammatory and apoptotic markers. These findings
suggest I. gabonensis as a potential natural therapy for male
reproductive damage caused by environmental toxins, warranting further
exploration of its clinical relevance and mechanisms.
Abstract Keywords
Irvingia gabonensis, lead toxicity,
oxidative stress, antioxidant, hormonal balance, male infertility, DNA fragmentation.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Editor-in-Chief
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).