Research Article
Pallab Kar
Pallab Kar
B.S. Diagnostic and Pathology Laboratory, Siliguri-734001, India. E-mail: pallabkar.bio@gmail.com
Abstract
Herbs are the core of traditional medicine. Several plants
have been identified and extensively utilized for their therapeutic value from
the very beginning of civilization. Clerodendrum
colebrookianum is a member of Lamiaceae family;
mostly they are perennial shrubs and in Indian Ayurvedic system they are used
in many of the herbal preparations. C.
colebrookianum are distributed in tropical
regions of Asia including India, Myanmar, Bangladesh, Malayasia, Indonesia,
Thailand, Bhutan, Nepal and also in temperate Tibet. Since the extracts
exhibited potent hepatoprotective, nephroprotective and anti-hypertensive
activity, it would be amicable to identify the active phytocompounds
responsible for those activities present in the extracts. In this regard, GC-MS
analysis has been considered. The A total number of six (6) phytocompounds have
been identified in C. colebrookianum leaves (CCL). Among the compounds, Linolenic acid, methyl ester
acid and hexadecanoic acid are some of the essential fatty acids that human
being requires in diet. The bioactive compounds of CCL were checked for
possible interactions with several proteins playing the essential role in
different metabolic pathways of humans and other major vertebrates. The
proteins were chosen those have relationship with the health of the liver.
These proteins acted as receptors required for molecular docking experiments.
The highest binding affinity (-8.7 kcal/mol) was found between Stigmasterol and
a protein with PDB ID 3I7H which is the crystal structure of DDB1 in complex
with H-Box Motif of HBX. Hence, present finding could open a new door to
understand the roots of several diseases and disorders facilitating new drug
discovery.
Abstract Keywords
Clerodendrum colebrookianum; GC-MS; hepatoprotective; molecular docking
Abstract
Herbs are the core of traditional medicine. Several plants
have been identified and extensively utilized for their therapeutic value from
the very beginning of civilization. Clerodendrum
colebrookianum is a member of Lamiaceae family;
mostly they are perennial shrubs and in Indian Ayurvedic system they are used
in many of the herbal preparations. C.
colebrookianum are distributed in tropical
regions of Asia including India, Myanmar, Bangladesh, Malayasia, Indonesia,
Thailand, Bhutan, Nepal and also in temperate Tibet. Since the extracts
exhibited potent hepatoprotective, nephroprotective and anti-hypertensive
activity, it would be amicable to identify the active phytocompounds
responsible for those activities present in the extracts. In this regard, GC-MS
analysis has been considered. The A total number of six (6) phytocompounds have
been identified in C. colebrookianum leaves (CCL). Among the compounds, Linolenic acid, methyl ester
acid and hexadecanoic acid are some of the essential fatty acids that human
being requires in diet. The bioactive compounds of CCL were checked for
possible interactions with several proteins playing the essential role in
different metabolic pathways of humans and other major vertebrates. The
proteins were chosen those have relationship with the health of the liver.
These proteins acted as receptors required for molecular docking experiments.
The highest binding affinity (-8.7 kcal/mol) was found between Stigmasterol and
a protein with PDB ID 3I7H which is the crystal structure of DDB1 in complex
with H-Box Motif of HBX. Hence, present finding could open a new door to
understand the roots of several diseases and disorders facilitating new drug
discovery.
Keywords
Clerodendrum
colebrookianum; GC-MS;
hepatoprotective; molecular docking
References
1. Mahishi, P.; Srinivasa, B.H.; Shivanna, M.B. Medicinal plant wealth of local communities in some villages in Shimoga District of Karnataka, India. J.
Ethnopharmacol. 2005, 98, 307-312.
2.
Boro, A.; Sarma, G.C. Ethnic uses of some wetland plants by
the Bodo community in Udalgiri district of Assam, India. Pleione 2013, 7,
155-159.
3.
Shil, S.; Choudhury, M.D.; Das, S. Indigenous knowledge of
medicinal plants used by the Reang tribe of Tripura state of India. J. Ethnopharmacol. 2014, 152,
135-141.
4.
Chandel, K.P.S.; Shukla, G.; Sharma, N. Biodiversity in
medicinal and aromatic plants in India: conservation and utilization. Indian
Council of Agricultural Research, 1996, NBPGR, Pusa Campus, New Delhi, India.
5.
Lokho, K.; Narasimhan, D. Ethnobotany of Mao-Naga Tribe of
Manipur, India. Pleione 2013, 7, 314-324.
6.
Kar, P.; Goyal, A.; Das, A.; Sen, A. Antioxidant and
pharmaceutical potential of Clerodendrum L.: an overview. Int. J. Green
Pharm. 2014, 8 (4), 210–216.
7.
Ahmed, A. Ethnobotanical studies on the Mishing tribes of
Assam with special reference to food and medicinal plants-1. J. Econ. Taxon. Bot. 1996, 12,
350-356.
8.
Nath, S.C.; Bordoloi, D.N. Clerodendrum colebrookianum, a folk remedy for the treatment of hypertension in
northeastern India. Int. J. Pharma. 1991, 29, 127-129.
9.
Das, S.; Vasudeva, N.; Sharma, S. Chemical composition of
ethanol extract of Macrotyloma uniflorum (lam.) Verdc. Using GC-MS
spectroscopy. Organ. Med. Chem. Lett. 2014, 4, 13.
10.
Trott, O.; Olson, A.J. AutoDock Vina: improving the speed
and accuracy of docking with a new scoring function, efficient optimization,
and multithreading. J. Comput. Chem. 2010, 31, 455.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
Herbs are the core of traditional medicine. Several plants
have been identified and extensively utilized for their therapeutic value from
the very beginning of civilization. Clerodendrum
colebrookianum is a member of Lamiaceae family;
mostly they are perennial shrubs and in Indian Ayurvedic system they are used
in many of the herbal preparations. C.
colebrookianum are distributed in tropical
regions of Asia including India, Myanmar, Bangladesh, Malayasia, Indonesia,
Thailand, Bhutan, Nepal and also in temperate Tibet. Since the extracts
exhibited potent hepatoprotective, nephroprotective and anti-hypertensive
activity, it would be amicable to identify the active phytocompounds
responsible for those activities present in the extracts. In this regard, GC-MS
analysis has been considered. The A total number of six (6) phytocompounds have
been identified in C. colebrookianum leaves (CCL). Among the compounds, Linolenic acid, methyl ester
acid and hexadecanoic acid are some of the essential fatty acids that human
being requires in diet. The bioactive compounds of CCL were checked for
possible interactions with several proteins playing the essential role in
different metabolic pathways of humans and other major vertebrates. The
proteins were chosen those have relationship with the health of the liver.
These proteins acted as receptors required for molecular docking experiments.
The highest binding affinity (-8.7 kcal/mol) was found between Stigmasterol and
a protein with PDB ID 3I7H which is the crystal structure of DDB1 in complex
with H-Box Motif of HBX. Hence, present finding could open a new door to
understand the roots of several diseases and disorders facilitating new drug
discovery.
Abstract Keywords
Clerodendrum colebrookianum; GC-MS; hepatoprotective; molecular docking
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).