Research Article
Cleiton de Oliveira¹, Camila Confortin¹, Wanderlei do Amaral¹, Ana
Helena Loos Moritz2, Michele Debiasi
Alberton² and Luiz Everson da Silva¹*
Abstract
Studies for the
knowledge of native species with therapeutic potential gain great relevance and
among these, there are researches with essential oils of native species. This
study aims to know the yield, chemical characterization and biological activity
of the essential oil of the species Eupatorium intermedium. The plant
material was collected in the Atlantic rainforest of the State of Paraná,
Brazil. The essential oil extraction was performed by hydrodistillation of the
inflorescences and leaves, in triplicates with 100g of plant material. The
yield obtained from the essential oil was 1.02% in the inflorescences and 0.74%
in the leaves. Gas chromatography analysis was performed on a Shimadzu
GCMS-TQ8040 brand, 90 division chromatographs coupled to a mass spectrometer.
The main constituents in flowers were α-pinene (25.88%), β-pinene (39.12%),
limonene (12.36%), 5-diene-trans-muurola-4(14) (4, 03%). On the other hands,
α-pinene (19.81%), β-pinene (25.93%), limonene (15.03%),
5-diene-trans-muurola-4(14) (10.55%), bicyclogermacrene (9.09%) were found in
the leaves. In vitro antibacterial assays were performed in triplicate
by agar diffusion method. The leaf oil generated a halo of inhibition with an
average of 14 mm for Listeria monocytogenes, Escherichia coli (16 mm), Bacillus
cereus (16 mm) and Staphylococcus aureus (17 mm). The inflorescence oil
generated a halo of inhibition with an average of 16 mm for Listeria
monocytogenes, Escherichia coli (13.66 mm), Bacillus cereus (13.66 mm),
Staphylococcus aureus (13.33 mm). In addition, the results of enzyme inhibition
assays indicated inhibition of α-glucosidase (100%) and acetylcholinesterase
(85.4%). These
results contribute to the chemical characterization of the species and are also
promising concerning the biological activity, being this the first ever report
of activity from the essential oil of the species Eupatorium intermedium
against the enzyme α-glucosidase and acetylcholinesterase.
Abstract Keywords
Essential oil; Eupatorium intermedium,
chemical composition, biological activities, medicinal plants, bioprospecting,
natural products, Asteraceae.
Abstract
Studies for the
knowledge of native species with therapeutic potential gain great relevance and
among these, there are researches with essential oils of native species. This
study aims to know the yield, chemical characterization and biological activity
of the essential oil of the species Eupatorium intermedium. The plant
material was collected in the Atlantic rainforest of the State of Paraná,
Brazil. The essential oil extraction was performed by hydrodistillation of the
inflorescences and leaves, in triplicates with 100g of plant material. The
yield obtained from the essential oil was 1.02% in the inflorescences and 0.74%
in the leaves. Gas chromatography analysis was performed on a Shimadzu
GCMS-TQ8040 brand, 90 division chromatographs coupled to a mass spectrometer.
The main constituents in flowers were α-pinene (25.88%), β-pinene (39.12%),
limonene (12.36%), 5-diene-trans-muurola-4(14) (4, 03%). On the other hands,
α-pinene (19.81%), β-pinene (25.93%), limonene (15.03%),
5-diene-trans-muurola-4(14) (10.55%), bicyclogermacrene (9.09%) were found in
the leaves. In vitro antibacterial assays were performed in triplicate
by agar diffusion method. The leaf oil generated a halo of inhibition with an
average of 14 mm for Listeria monocytogenes, Escherichia coli (16 mm), Bacillus
cereus (16 mm) and Staphylococcus aureus (17 mm). The inflorescence oil
generated a halo of inhibition with an average of 16 mm for Listeria
monocytogenes, Escherichia coli (13.66 mm), Bacillus cereus (13.66 mm),
Staphylococcus aureus (13.33 mm). In addition, the results of enzyme inhibition
assays indicated inhibition of α-glucosidase (100%) and acetylcholinesterase
(85.4%). These
results contribute to the chemical characterization of the species and are also
promising concerning the biological activity, being this the first ever report
of activity from the essential oil of the species Eupatorium intermedium
against the enzyme α-glucosidase and acetylcholinesterase.
Keywords
Essential oil; Eupatorium intermedium,
chemical composition, biological activities, medicinal plants, bioprospecting,
natural products, Asteraceae.
References
1
Lima, H. R. P.; Kaplan, M. A. C.; Cruz,
A. V.M. de. Influência dos Fatores Abióticos na Produção e Variabilidade de
Terpenóides em Plantas. Floresta e Ambiente 2003, 10, 71-77.
2
Santos, R. I. Metabolismo básico e
origem dos metabólitos secundários. In: Farmacognosia da planta ao medicamento,
Simões, C. M. O.; Schenkel, E. P.; Gosmann, G.; Mello, J. C. P; Mentz, L. A.;
Petrovick, P.R., Editor. 2004, Editora da UFSC: Porto Alegre, RS. p. 323-354.
3
Solórzano-Santos, F.; Miranda-Novales, M.G. Essential
oils from aromatic herbs as antimicrobial agents. Current Opinion Biotechnol. 2011, 23, 1-6.
4
StanskI, C.; Nogueira, M.K.F.S.; Da Luz,
C.F.P. Palinologia de espécies de Asteraceae de utilidade medicinal para a
região dos Campos Gerais, Ponta Grossa, PR, Brasil. Hoehnea 2016, 43, 349-360.
5
Varjabedian, R. Lei da Mata Atlântica:
retrocesso ambiental. Estudos avançados 2010,
24, 147-160.
6
Amaral, W. Prospecção de espécies
aromáticas silvestres dos Campos Gerais da Mata Atlântica do Paraná. Tese
(Doutorado) do Programa de Pós-Graduação em Agronomia, Produção Vegetal. 137 f.
Curitiba, 2015.
7
Girard, E. A.; Koehler, H. S; Netto,
S.P. Volume, biomassa e rendimento de óleos essenciais do craveiro (pimenta pseudocaryophyllus
(gomes) landrum). Rev. Acad. 2007,
5(2), 147-165.
8
Santos, T.G.; Fukuda, K.; Kato, M.J.; SArtorato, A.;
Duarte, M.C.T.; Ruiz, A.L.T.G.; Carvalho, J.E.; De, Augusto F.; Marques, F.A.;
Maia, B.H.L.N.S. Characterization of the essential oils of two species of
Piperaceae by one- and two-dimensional chromatographic techniques with
quadrupole mass spectrometric detection. Microchem. J. 2014, 115, 113–120.
9
Adams, R. P. Identification of essential oil
components by gas chromatography/mass spectrometry, ed. 4.1, Allured Pub. Corp.
2017.
10
Ostrosky, E. A.; Mizumoto, M.K.; Lima,
M.E.L.; Kaneko, T.M.; Nishikawa, S.O.; Freitas, B.R. Métodos para Avaliação da
Atividade Antimicrobiana e Determinação da Concentração Mínima Inibitória (CMI)
de Plantas Medicinais. Rev. Bras. Farmacog. 2008, 18, 301–307.
11
Silva, L.E.; Gonçalves, M.V.S.; Do
Amaral, W.; De Quadros, D.A.; Reis, R.A.; Do Amaral, L.D.P.; Huergo, L.F.;
Gracia, B. Chemical composition and antibacterial activity of Cymbopogon
citratus and Cymbopogon flexuosus essential oils. Ciência e Natura 2018, 40, e2.
12
Kim, Y. M.; Wang, M. H.; Rhee, H. I. A novel
alpha-glucosidase inhibitor from pine bark. Carbohydrate Res.
2004, 339(3), 715–717.
13
Mata, A.T.; Proença, C.; Ferreira, A.R.;
Serralheiro, M.L.M.; Nogueira, J.M.F.; Araújo, M.E.M. Antioxidant and
antiacetylcholinesterase activities of five plants used as portuguese food
spices. Food Chem. 2007,
103, 778-786.
14
ApelL, M.A.; Souza, T.J.T.; Andrade,
J.M.M.; Bordignon, S.A.L.; Matzembacher, N.I. Henriques, A.T. Eupatorium
rufescens, E. grande e E. consanguineum: avaliação preliminar
das atividades antioxidante e anticolinesterásica e determinação da composição
química do óleo volátil. 31a Reunião Anual da Sociedade Brasileira de Química,
2008.
15
Czaikoski, k.; Mesomo, M.C.; Scheer,
A.D.P.; Queiroga, C.L.; Deschamps, C.; Corazza, M.L. Composição química e
atividade biológica do óleo essencial de Eupatorium intermedium. J.
Essent. Oil Res. 2017, 29(1), 93 -100. DOI: 10.1080/10412905.2016.1191382
16
Pimienta-Ramírez, L.; García-Rodríguez,
Y.M.; Ríos-Ramírez, E.M.; Lindig-Cisneros, R.; Espinosa-García, F.J. Composição
química e avaliação do óleo essencial de Eupatorium glabratum como
biopesticida contra Sitophilus zeamais e vários milhos armazenados
fungi. J. Essent. Oil Res. 2016,
28(2), 113-120. DOI: 10.1080/10412905.2015.1093969
17
Souza, T.J. T.; Apel, M.A.; Bordignon,
S.; Matzenbacher, N.I.; Zuanazzi, J.A.S. Composição química e atividade
antioxidante do óleo volátil de Eupatorium polystachyum DC. Rev. Bras.
Farmacog. 2007, 17(3), 368-372.
18
Alzoreky, N. S.; Nakahara, K. Antibacterial activity
of extracts from some edible plants commonly consumed in Asia. Inter. J.
Food Microbiol. 2003,
80 (3), 223-230.
19
Rabêlo, W.F. Caracterização química,
Toxicidade e avaliação da atividade antibacteriana do óleo essencial do cravo
da índia (Syzygium aromaticum). Dissertação (Mestrado). Universidade
Federal do Maranhão, 2010.
20
Sugumar, N.; Karthikeyan, S.; Gowdhami,
T. Composição química e atividade antimicrobiana do óleo essencial de Eupatorium
triplinerve Vahl. peças aéreas. Int. Lett. Nat. Sci. 2015,
4, 14–21.
21
Monteiro, J.S.M. Valorização de plantas
aromáticas catarinenses – estudo químico-biológico dos óleos essenciais de Piper
mikanianum (KUNTH) STEUDEL e Drimys angustifolia MIERS. 91f.
Dissertação (Mestrado em Química) - Programa de Pós-Graduação em Química,
Centro de Ciências Exatas e Naturais, Universidade Regional de Blumenau,
Blumenau, 2018.
22
Alcântara, J.M.; Yamaguchi, K.K. L.;
Silva, J.R.A.; Veiga Junior, V.F. Composição química e atividade biológica dos
óleos essenciais das folhas e caules de Rhodostemonodaphne parvifolia
Madriñán (Lauraceae). Acta Amazônica 2010, 40(3), 567 – 572.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
Studies for the
knowledge of native species with therapeutic potential gain great relevance and
among these, there are researches with essential oils of native species. This
study aims to know the yield, chemical characterization and biological activity
of the essential oil of the species Eupatorium intermedium. The plant
material was collected in the Atlantic rainforest of the State of Paraná,
Brazil. The essential oil extraction was performed by hydrodistillation of the
inflorescences and leaves, in triplicates with 100g of plant material. The
yield obtained from the essential oil was 1.02% in the inflorescences and 0.74%
in the leaves. Gas chromatography analysis was performed on a Shimadzu
GCMS-TQ8040 brand, 90 division chromatographs coupled to a mass spectrometer.
The main constituents in flowers were α-pinene (25.88%), β-pinene (39.12%),
limonene (12.36%), 5-diene-trans-muurola-4(14) (4, 03%). On the other hands,
α-pinene (19.81%), β-pinene (25.93%), limonene (15.03%),
5-diene-trans-muurola-4(14) (10.55%), bicyclogermacrene (9.09%) were found in
the leaves. In vitro antibacterial assays were performed in triplicate
by agar diffusion method. The leaf oil generated a halo of inhibition with an
average of 14 mm for Listeria monocytogenes, Escherichia coli (16 mm), Bacillus
cereus (16 mm) and Staphylococcus aureus (17 mm). The inflorescence oil
generated a halo of inhibition with an average of 16 mm for Listeria
monocytogenes, Escherichia coli (13.66 mm), Bacillus cereus (13.66 mm),
Staphylococcus aureus (13.33 mm). In addition, the results of enzyme inhibition
assays indicated inhibition of α-glucosidase (100%) and acetylcholinesterase
(85.4%). These
results contribute to the chemical characterization of the species and are also
promising concerning the biological activity, being this the first ever report
of activity from the essential oil of the species Eupatorium intermedium
against the enzyme α-glucosidase and acetylcholinesterase.
Abstract Keywords
Essential oil; Eupatorium intermedium,
chemical composition, biological activities, medicinal plants, bioprospecting,
natural products, Asteraceae.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Editor-in-Chief
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).