Abstract

A total of 120 maize inbred lines were subjected to genetic diversity analysis by 40 SSR markers. The study revealed total alleles of 146 across 40 simple sequence repeat (SSR) markers, ranged from 2 to 8 with the average of 4 per locus. Gene diversity was observed with the mean value of 0.55, range from 0.11 to 0.71 among maize genotypes. Polymorphism Information Content (PIC) value ranged from 0.10 to 0.76 with the mean value of 0.48. Umc1525 was the best marker in this study for identification of genotypes as revealed by its PIC values of 0.76. A wide range of genetic variability and PIC value were observed among maize genotypes designating that tested genotypes are invaluable genetic materials for the breeding program. In UPGMA classification by molecular markers, the genotypes were identified three distinct clusters. The log-likelihood exposed by the population structure with the optimum K value of 4, pointed out that genotypes could be divided into four sub-populations. Genotypes involved in sub-population IV analyzed by population structure, which was one group in cluster IIIB constructed by UPGMA, indicating that genotypes in sub-population IV were unique and diverse from other maize genotypes. Principal coordinate analysis (PCoA) was performed to identify maize inbred lines into genetically diverged and similar genotypes. Based on this finding, genotypes viz. TK.19.17, TK.19.20, TK.19.21, TK.19.27, TK.19.28, TK19.31, TK.19.33, YZSI.20.006, YZSI.20.15, YZSI.20.16, YZSI.20.17, YZSI.20.26, YZSI.20.054, YZSI.20.036 and YZSI.20.027 were genetically diverged and useful in maize breeding program for the exploitation of heterosis.

Keywords

Genetic diversity; maize inbred lines; SSR markers; PIC; clusters, population structure

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