Research Article
Abstract
Clerodendrum is
a member of Lamiaceae family; mostly they are perennial shrubs and in Indian
Ayurvedic system they are used in many of the herbal preparations. Species of Clerodendrum
are distributed in tropical regions of Asia including India, Myanmar,
Bangladesh, Malayasia, Indonesia, Thailand, Bhutan, Nepal and also in temperate
Tibet. Moreover, the medicinal properties of the species are attributed to
their phytochemical constituents that are believed to cure varied ailments.
Although, ethnobotanically, they have diverse medicinal properties but there is
no sufficient information illustrating the comprehensive genetic variation
among the different medicinal species of Clerodendrum. Hence, an initiative was
undertaken to discriminate the genetic variations among 11 important species of
Clerodendrum through DNA barcoding techniques. The DNA barcode analysis by
means of matK, Rps16 and TrnL-F clearly reflected that two subfamily
Symphorematoideae and Nepetoideae very close to Ajugoideae which validates the
traditional classification of Cronquist.
Keywords
Clerodendrum; matK; Rps16; TrnL-F; DNA barcode
Abstract
Clerodendrum is
a member of Lamiaceae family; mostly they are perennial shrubs and in Indian
Ayurvedic system they are used in many of the herbal preparations. Species of Clerodendrum
are distributed in tropical regions of Asia including India, Myanmar,
Bangladesh, Malayasia, Indonesia, Thailand, Bhutan, Nepal and also in temperate
Tibet. Moreover, the medicinal properties of the species are attributed to
their phytochemical constituents that are believed to cure varied ailments.
Although, ethnobotanically, they have diverse medicinal properties but there is
no sufficient information illustrating the comprehensive genetic variation
among the different medicinal species of Clerodendrum. Hence, an initiative was
undertaken to discriminate the genetic variations among 11 important species of
Clerodendrum through DNA barcoding techniques. The DNA barcode analysis by
means of matK, Rps16 and TrnL-F clearly reflected that two subfamily
Symphorematoideae and Nepetoideae very close to Ajugoideae which validates the
traditional classification of Cronquist.
Keywords
Clerodendrum; matK; Rps16; TrnL-F; DNA barcode
References
1. Rueda, R.M.
The genus Clerodendrum (verbenaceae) in Mesoamerica. Ann. Mo. Bot. Gard. 1993, 870-890.
2. Hsiao, J.Y.;
Lin, M.L. A chemotaxonomic study of essential oils from the leaves of genus Clerodendrum
(Verbenaceae) native to Taiwan. Bot. Bull.
Acad. Sin. 1995, 36, 247-251.
3. Moldenke, H.N.
Notes on the genus Clerodendrum (Verbenaceae): 4. Phytologia 1985, 57, 334-365.
4. Steane, D.A.;
Scotland, R.W.; Mabberley, D.J.; Olmstead, R.G. Molecular systematics of Clerodendrum (Lamiaceae):
ITS sequences and total evidence. Am. J. Bot. 1999, 86, 98-107.
5. Shrivastava,
N.; Patel, T. Clerodendrum and heathcare: an overview. Aromat Plant Sci Biotechnol. 2007, 1, 142-150.
6. Rajendran, A.;
Daniel, P. The Indian Verbenaceae: A Taxonomic Revision. Bishen Singh Mahendra
Pal Singh, Dehradun, 2002.
7. Srivastava,
R.C.; Choudhary, R.K. Species diversity and Economic importance of the family
Verbenaceae in Arunachal Pradesh. Bull. Arunachal
Pradesh Res. 2008, 24, 1-21.
8. Fletcher, H.R.
The Siamese Verbenaceae. Bull. Misc. Inform. (Royal Botanic Gardens, Kew) 1938, 1938, 401-445.
9. Kochummen,
K.M. Verbenaceae. In: F.S.P. Ng (Ed.), Tree Flora of Malaya, vol. 3, Singapore:
Multiprint Services, 1978, 297-313.
10. Liang, C.S.;
Gilbert, M.G. Verbenaceae. In W.Z. Yi and P.H. Raven (Eds.), Flora of China,
vol. 17, Science Press, Missouri, Botanical Garden, 1984, 34-43.
11. Munir, A.A. A
taxonomic revision of the genus Clerodendrum L.(Verbenaceae) in Australia. J. Adel. Bot. Gard. 1989, 101-173.
12. Cantino, P.D.;
Harley, R.M.; Wagstaff, S.J. Genera of Labitae: Status and Classification. In
R.M. Harley and T. Reynolds (Eds.). Advances in Labiate Science. Royal Botanic
Gardens, Kew, 1992, 511-523.
13. Harley, R.M.;
Atkins, S.; Budantsev, P.D. The Families and Genera of Vascular Plants:
Flowering Plant-Dicotyledons. Labiatae. Germany: Springer-Verlag 2004, 267-8.
14. Hebert,
P.D.N.; Cywinska, A.; Ball, S.L. Biological identifications through DNA
barcodes. Proc. R. Soc. B: Biol. Sci. 2003, 270, 313-321.
15. Doyle, J.J.;
Doyle, J.L. A rapid DNA isolation procedure for small quantities of fresh leaf
tissue. Phytochem. Bull. 1987, 19, 11-15.
16. Tamura, K.;
Dudley, J.; Nei, M.; Kumar, S. MEGA4: molecular evolutionary genetics analysis
(MEGA) software version 4.0. Mol. Biol.
Evol. 2007, 24, 1596-1599.
17. Saitou, N.;
Nei, M. The neighbor-joining method: a new method for reconstructing
phylogenetic trees. Mol. Biol. Evol. 1987, 4, 406-425.
18. Cronquist, A.
An integrated system of classification of flowering plants. Columbia University
Press, New York, 1981.
19. Schäferhoff,
B.; Fleischmann, A.; Fischer, E.; Albach, D.C.; et al. Towards resolving
Lamiales relationships: insights from rapidly evolving chloroplast sequences. BMC Evol. Biol. 2010, 10, 352.
20. Selvaraj, D.;
Sarma, R.K.; Sathishkumar, R. Phylogenetic analysis of chloroplast matK gene
from Zingiberaceae for plant DNA barcoding. Bioinformation 2008, 3, 24.
This work is licensed under the
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License (CC BY-NC 4.0).
Abstract
Clerodendrum is
a member of Lamiaceae family; mostly they are perennial shrubs and in Indian
Ayurvedic system they are used in many of the herbal preparations. Species of Clerodendrum
are distributed in tropical regions of Asia including India, Myanmar,
Bangladesh, Malayasia, Indonesia, Thailand, Bhutan, Nepal and also in temperate
Tibet. Moreover, the medicinal properties of the species are attributed to
their phytochemical constituents that are believed to cure varied ailments.
Although, ethnobotanically, they have diverse medicinal properties but there is
no sufficient information illustrating the comprehensive genetic variation
among the different medicinal species of Clerodendrum. Hence, an initiative was
undertaken to discriminate the genetic variations among 11 important species of
Clerodendrum through DNA barcoding techniques. The DNA barcode analysis by
means of matK, Rps16 and TrnL-F clearly reflected that two subfamily
Symphorematoideae and Nepetoideae very close to Ajugoideae which validates the
traditional classification of Cronquist.
Abstract Keywords
Clerodendrum; matK; Rps16; TrnL-F; DNA barcode
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).